Buchholz, Frank, Prof. Dr. rer. nat.
NCT/UCC, Medical Systems Biology
University Hospital Carl Gustav Carus
Technische Universität Dresden (TUD)
Professor of Medical Systems Biology (since 2010)
Head of Translational Research, National Center for Tumor Diseases (NCT)/University Cancer Center (UCC)
Role within partner site
Translational research in the field of genome editing and gene therapy;
Relevant work experience
Molecular biologist with focus on systems biology and gene editing;
2019– Scientific Speaker of the Mildred-Scheel-Nachwuchszentrum Dresden
2017– Member of the Director’s Advisory Board (DAB) of the Institute of Cancer Research, MBICR China
2010– Professor of Medical Systems Biology, TUD
2010– Head of Translational Research, NCT/UCC
2002–2010 Group Leader, MPI-CBG Dresden
1998–2001 Postdoctoral research in the field of Molecular Biology and Genetics, University of California San Francisco, CA, USA
1997–1998 Postdoctoral research in the field of Molecular Biology and Genetics, EMBL Heidelberg
1994–1997 PhD in Molecular Biology, EMBL Heidelberg
1987–1993 Diploma in Molecular Genetics, University of Göttingen and University of California San Diego, CA, USA
Main research focus
Development and implementation of gene editing tools, genome surgery, functional genomics, cancer and viral diseases research;
5 out of 153; SCOPUS h-index 47
Gebler C, …, Buchholz F (2017). Inactivation of cancer mutations utilizing CRISPR/Cas9. J Natl Cancer Inst 109:djw183. doi: 10.1093/jnci/djw183.
Karpinski J, …, Buchholz F (2016). Directed evolution of a recombinase that excises the provirus of most HIV-1 primary isolates with high specificity. Nat Biotechnol 34:401–409.
Karimova M, …, Buchholz F (2016). Discovery of Nigri/nox and Panto/pox site-specific recombinase systems facilitates advanced genome engineering. Sci Rep 6:30130. doi: 10.1038/srep30130.
Sarkar I, …, Buchholz F (2007). HIV-1 proviral DNA excision using an evolved recombinase. Science 316:1912–1915.
Buchholz F and Stewart AF (2001). Alteration of Cre recombinase site specificity by substrate-linked protein evolution. Nat Biotechnol 19:1047–1052.
EP2094855, EP2576798, EP2690177, EP1537227, EP1348028, EP1034260, EP1291420, EP2877585, EP17175895;
Awards: GO-Bio Prize, BMBF (2018 & 2008), Heinz-Ansmann Prize (2017), Bayer Healthcare Hemophilia Award (2015);
Grants: ERC Advanced Grant (2018);
Memberships: EMBO member (2016);